Post-Doc H/F - Sanofi

Post-Doc Position in Sanofi / WIDGET consortium

Expert in Biology, Molecular Biology, NGS & Single Cells

Abstract :

As part of the WIDGeT consortium1 (23M€, 5 years), we are currently researching a Postdoctoral candidate with a strong background in molecular biology and NGS for single cell application.

Overview of the research project :

The WIDGeT consortium (Viral Vector Intelligent Design for Gene Therapy) aims to accelerate the development of gene therapies using viral vectors derived from adeno-associated viruses (AAV) for the treatment of age-related macular degeneration and hereditary podocytopathies (kidney diseases), leveraging the potential of artificial intelligence (AI). As part of this challenging consortium, our team proposed to develop a new technology to provide very sensitive information of AAV's transgene expression at the single cell level. This technology is based on droplet microfluidic, DNA barcoded, scRT-PCR and NGS.

Responsibilities :

·Develop, design, and execute complex experiments and analyze data.

·Write (Lab Book) and Present (PowerPoint) research findings.

·Mentor and co-supervise engineer, technician and undergraduate students involved in the research project.

·Report and collaborate with other members of the consortium.

Qualifications :

·Ph.D. in biology or molecular biology

·Strong Experience in Single-cell RNA sequencing

·Strong background in molecular biology, NGS experiments and analysis.

·Background in single cells experiment (10x, flow cytometer...)

·Appetite for bench work & wet lab

·Appetite for challenging project

·Background in Bioinformatic

·Familiarity with statistical software (Prism, Spotfire, other...)

·Fluent in English

·Excellent written and verbal communication skills.

·Experience with Recombinant AAV is a plus.

·Knowledge in organoids/tissues preparation is a plus.

·Experience in screening is a plus.

·Background in Droplet microfluidic is optional.

The Project Team :

A dedicated team was created in 2024 to tackle this challenging project. The team is composed of one researcher, one engineer and one technician.

The Lab :

Our innovative Team (~8 people) is based in Vitry-Sur-Seine (Suburbs of Paris) at Sanofi R&D site. We have two laboratories (L1 & L2) dedicated to droplet microfluidic technology, with access to diverse technologies (ARIA Fusion (FACS), PCR, qPCR and NextSeq Illumina).

Duration & Salary :

18 months to 4 years

Language :

English / French (optional)

References :

1

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Dans le cadre de son engagement diversité, Sanofi accueille et intègre des collaborateurs en situation de handicap.