Scientist - Proteomics W - M H/F - Lesaffre

As a global key player in yeasts and fermentation, Lesaffre designs, manufactures and markets innovative solutions forBaking, Food taste & pleasure, Health care and Biotechnology.

Family group born in northern France in 1853, now a multi-national and a multicultural company, Lesaffre is committed toworking with confidence to better nourish and protect the planet.
In close collaboration with its clients and partners, Lesaffre employs11 000 peoplein more than50 countries.

Lesaffre achieves a turnover of 3 billion Euros.Part of the Analytical Science and Discovery team, proteomics is a recently established activity. With a view of activity growth, we are looking for a scientist (PhD) specialized in proteomics. You will report to the Head of Metabolomics/Proteomics Operations and contribute to the success of R&D projects through the proteome/peptidome characterization necessary for the development of new processes and new products.

The successful candidate will BE responsible for developing, adapting and applying proteomics workflows to various projects from study design, sample preparation, LC-MSMS analyses, to data processing and interpretation, as well as proteomics results presentation. You will work in a multidisciplinary environment. We are looking for a motivated and dynamic candidate with solid technical and organizational skills, coupled with a strong collaborative spirit.

Responsabilities
- Define, plan and perform experiments : sample preparation, LC-MS/MS method setting for data acquisition, data treatment and interpretation with results validation
- Organize and manage research data using an electronic laboratory notebook.
- Respect deadlines, demonstrate scientific initiative by revising and adapting experiments based on the requests and expected results.
- Responsible for equipment management (implementation, maintenance, follow-up procedures)
- Propose and implement new innovative technologies/methodologies, improving proteomic current workflows and throughput via automation
- Communicate the results, report on your activity and participate in the alignment of experimental activities and project needs.
- Contribute to the general organizational tasks of the laboratory such as the inventory of consumables, functional control of equipment, etc.
- Contribution to information sharing and communication within the analytical team as well as the various interdisciplinary project-based groups.

- PhD in analytical chemistry or biochemistry, specialized in the analysis of proteins and peptides using mass spectrometry.
- You have significant first professional experience in proteomics ideally applied in the domain of system biology approach to analyze the dynamics of proteins in living systems.
- Experience in sample preparation for proteomics approaches including subcellular fractionation, protein extraction and purification, dosage, protein enrichment and separation techniques, optimization of enzymatic digestion, peptide desalting, fractionation, and enrichment when required.
- Experience in handling high-resolution mass spectrometers (specifically Thermo Orbitrap instruments) to define adapted acquisition methods.
- Familiar with proteomics softwares and tools for untargeted (Proteome Discoverer, Peaks software) and targeted (Skyline) analyses, as well as other bioinformatics tools and databases. Experience in both qualitative and quantitative proteomics.
- Familiar with tools related to data curation, validation and interpretation, with summing up into the main conclusions.
- Good communication and writing skills, especially in English.
- Good general knowledge of organic chemistry and biochemistry.