Researcher In Optical Instrumentation For Cryogenic Super-Resolution Microscopy Cryo-Palm H/F - CEA
Les missions du poste
Il apporte des solutions concrètes à leurs besoins dans quatre domaines principaux : transition énergétique, transition numérique, technologies pour la médecine du futur, défense et sécurité sur un socle de recherche fondamentale. Le CEA s'engage depuis plus de 75 ans au service de la souveraineté scientifique, technologique et industrielle de la France et de l'Europe pour un présent et un avenir mieux maîtrisés et plus sûrs.
Implanté au coeur des territoires équipés de très grandes infrastructures de recherche, le CEA dispose d'un large éventail de partenaires académiques et industriels en France, en Europe et à l'international.
Les 20 000 collaboratrices et collaborateurs du CEA partagent trois valeurs fondamentales :
- La conscience des responsabilités
- La coopération
- La curiosité
Would you like to develop optical instrumentation and methods for microscopy at very low temperatures? Join us as a researcher on a three-year fixed-term contract within the ANR EPIC project. You will investigate and optimise a cryo-PALM microscope and develop reliable cryogenic sample transfers between fluorescence and electron microscopy at IBS/IRIG!
As a researcher, your responsibilities will include:
- Develop and evaluate optical improvements to the cryo-PALM microscope: excitation and emission paths, photon collection efficiency, spatial sampling, optical alignment and quantitative point spread function (PSF) characterisation.
- Investigate and optimise illumination and acquisition strategies for cryo-FIB lamellae: spatially targeted activation/excitation, control of sample heating and improved single-molecule localisation precision.
- Develop and validate procedures for transferring vitrified samples between cryo-FIB/SEM, cryo-PALM and cryo-electron microscopy, maintaining cryogenic conditions and limiting contamination and deformation of the lamellae.
- Establish calibration methods and assess the performance of the correlative cryo-PALM/cryo-ET workflow with cryo-EM and image processing specialists; contribute to the evaluation of fluorescent proteins within the project.
Le profil recherché
We primarily seek a specialist in experimental optics or photonics with a PhD in optics, photonics, experimental physics, optical instrumentation or a related field. Hands-on experience designing, aligning and optimising optical or microscopy setups is essential. Knowledge of biophysics or biological applications is an asset; you will work closely with specialists in biology and cryo-EM. Prior PALM or cryo-EM experience is welcome but is not required.
Technical skills
Strong expertise in experimental optics, photonics and optical instrumentation.
Hands-on experience designing, aligning, optimising and quantitatively characterising optical or microscopy setups.
Knowledge of fluorescence microscopy, super-resolution imaging, cryogenic instrumentation or biological applications would be an asset.
Programming and quantitative experimental data analysis skills would be an asset.
Interpersonal skills
Independence, scientific rigour and good organisational skills.
Initiative, scientific curiosity and the ability to propose solutions to experimental challenges.
Ability to work as part of a team and collaborate in a multidisciplinary environment.
Good communication skills and the ability to share scientific results.
Language skills
Good command of spoken and written scientific English to work in an international environment.
Knowledge of French is welcome but is not essential.
🤝 Why join us? ....
By joining the Grenoble Interdisciplinary Institute, you'll be part of a dynamic and innovative research environment where you'll have the opportunity to learn, grow and play a key role within a centre of excellence.
...AND THAT'S NOT ALL!
You may be interested in the benefits that come alongside your main role:
A cutting-edge research ecosystem dedicated to topics of significant societal importance.
Training to enhance your skills and boost your career.
A staff committee offering a wealth of social, cultural and sporting benefits.
Practical benefits: staff canteen/meal vouchers, 85% of transport costs covered, and much more.
An exceptional living environment in Grenoble, nestled in the mountains, offering a unique balance between city life and nature.
🚀 Want to be part of the adventure? Apply now and put your energy to work for research and innovation!
In line with the CEA's commitments to the integration of people with disabilities, this role is open to everyone. The CEA offers adjustments and/or organisational arrangements to facilitate the inclusion of disabled workers
